RutinForomo ea lik'hemik'hale ke (C27H30O16•3H2O), vithamine, e na le phello ea ho fokotsa ho kenella ha capillary le ho robeha ha eona, ho boloka le ho tsosolosa ho tenyetseha ho tloaelehileng ha capillaries. Bakeng sa thibelo le kalafo ea ho tsoa mali maling a bokong ka lebaka la khatello e phahameng ea mali; Ho tsoa mali maling ha retina ka lebaka la lefu la tsoekere le purpura ea mali le tsona li sebelisoa e le li-antioxidants le mebala ea lijo.

E arotsoe ka lintlha tse 'ne tse latelang:

1. Rutin NF11: phofo e mosehla-botala, kapa kristale e ntle haholo ea acicular; Ha e na monko, ha e na tatso; 'Mala o fifala moeeng; Ha e futhumatsoa ho fihlela ho 185-192 ℃, e fetoha 'mele o sootho oa gelatinous 'me ea bola hoo e ka bang 215 ℃. E qhibiliha hanyane ka har'a ethanol e belang, e qhibiliha hanyane ka metsing a belang, e qhibiliha hanyane ka metsing a batang, e qhibiliha habonolo ka har'a isopropyl alcohol le methanol, ha e qhibilihe ka har'a trichloromethane, ether le benzene; E qhibiliha ka har'a tharollo ea alkali hydroxide. Mokhoa oa ho khetholla ke A: hydrochloric acid reflux hydrolysis ho quercetin, eo ntlha ea eona ea ho qhibiliha e leng 312 ℃ B: precipitation ea cuprous oxide e khubelu. C: Ho eketsa tharollo ea sodium hydroxide ke mosehla oa lamunu D: tharollo ea ethanol le tharollo ea ferric chloride ke sootho e tala E: tharollo ea ethanol e nang le hydrochloric acid le magnesium butle-butle e khubelu: ≥95.0%(UV)(ka lihlahisoa tse omileng)

Ho theola boima ba 'mele ka omileng: 5.5% ~ 9.0%

Masalla a tukang ≤0.5%

Chlorophyll ≤0.004%

Mmala o mofubelu ≤0.004%

Sehlahisoa se amanang le quercetin ≤5.0% (UV)

Palo yohle ya dibaktheria tsa aerobic ≤103cfu/g

Palo yohle ya hlobo le tomoso ≤102cfu/g

Escherichia coli e ke ke ea fumanoa /g

Maemo a polokelo Boloka ka setshelong se sa keneng moya hole le lesedi.

2. Rutoside Trihydrate EP 9.0: Phofo e mosehla kapa e mosehla-botala. E batla e sa qhibilihe metsing, e qhibiliha ka har'a methanol, e qhibiliha hanyane ka har'a ethanol (96%), e batla e sa qhibilihe ka har'a methylene chloride. E qhibiliha ka har'a tharollo ea hydroxide. Mokhoa oa ho khetholla ke o latelang: A: Ho monngoa ho hoholo ho 257nm le 358nm, 'me coefficient e phahameng ka ho fetisisa ea ho monngoa ho 358nm ke 305 ~ 330. B: Paterone ea ho monngoa ha infrared e lokela ho lumellana le ea sehlahisoa sa referense C: Matheba a 'mala o tšoanang le boholo a tla hlaha sebakeng se tsamaellanang sa chromatogram ea sehlahisoa sa referense D: tharollo ea ethanol e nang le hydrochloric acid le zinc e tla bontša bofubelu

Dikahare 95.0% ~ 101.0% (ka sehlahisoa se omileng)(titration)

Mongobo 7.5% ~ 9.5% (Cartesian)

Masalla a tukang ≤0.1%

Boleng bo phahameng ka ho fetisisa ba ho monya khanya ea litšila tsa optical ho tloha ho 450nm ho isa ho 800nm ​​ha boa lokela ho feta 0.10

Ntho e sa qhibiliheng ka har'a methanol ≤3.0%

Sehlahisoa se amanang le sona ke isoquercetin ≤2.0%, kaempferol-3-rutin ≤2.0%, quercetin ≤2.0%, ho se hlweke ho felletseng ≤4.0% (HPLC)

Palo yohle ya dibaktheria tsa aerobic ≤104cfu/g

Palo yohle ya hlobo le tomoso ≤102cfu/g

Libaktheria tse se nang gram ea bile ≤102cfu/g

Escherichia coli e ke ke ea fumanoa /g

Salmonella e kanna ea se ke ea fumanoa /25g

Maemo a polokelo a boloke hole le lesedi

3. Rutin USP43: Mokhoa oa ho khetholla ke A: ho monya ho hoholo ho 257nm le 358nm, 'me coefficient e phahameng ea ho monya ho 358nm ke 305 ~ 33. B: Li-spectra tsa infrared absorption li lokela ho lumellana le chromatogram ea sehlahisoa sa referense. C: Nako ea ho boloka ea tlhoro ea chromatogram e lokela ho lumellana le ea sehlahisoa sa referense.

Dikahare 95.0% ~ 101.0% (ka sehlahisoa se omileng)(titration)

Mongobo 7.5% ~ 9.5% (Cartesian)

Masalla a tukang ≤0.1%

Boleng bo phahameng ka ho fetisisa ba ho monya khanya ea litšila tsa optical ho tloha ho 450nm ho isa ho 800nm ​​ha boa lokela ho feta 0.10

Ntho e sa qhibiliheng ka har'a methanol ≤3.0%

Lintho tse amanang le isoquercetin ≤2.0%, kaempferol-3-rutin ≤2.0%, quercetin ≤2.0%, tse ling tse fapaneng tse 'ngoe ≤1.0%, ho se hloeke ho felletseng ≤4.0% (HPLC)

Palo yohle ya dibaktheria tsa aerobic ≤104cfu/g

Palo yohle ya hlobo le tomoso ≤103cfu/g

Escherichia coli e ke ke ea fumanoa /10g

Salmonella e ke ke ea fumanoa /10g

Boemo ba polokelo bo koetsoe 'me bo bolokiloe hole le khanya.

4. Tekanyetso ea Lekala WS1-49(B)-89 ea Rutinum: phofo e mosehla kapa e mosehla-botala, kapa kristale e ntle haholo ea acicular; Ha e na monko, ha e na tatso; 'Mala o fifala moeeng; E futhumatsoa ho fihlela ho 185 ~ 192℃ ho fetoha gel e sootho. E qhibiliha hanyane ka har'a ethanol e belang, e qhibiliha hanyane ka metsing a belang, e qhibiliha hanyane ka metsing a batang, ha e qhibilihe ka har'a trichloromethane le ether; E qhibiliha hanyane ka har'a tharollo ea alkali hydroxide. Mokhoa oa ho khetholla ke: A: red cuprous oxide precipitate. B: Paterone ea infrared ea ho monya e lokela ho lumellana le ea ntho e laoloang. C: Ho monya ho hoholo ho fumanoa maqhubung a 259±1nm le 362.5±1nm.

Dikahare ≥93.0%(UV)(ka sehlahisoa se omileng)

Ho theola boima ba 'mele ka omileng 5.5% ~ 9.0%

Masalla a tukang ≤0.3%

Ntho e sa qhibiliheng ka har'a methanol ≤2.5% (ntho e sa qhibiliheng ka har'a ethanol)

Sehlahisoa se amanang le quercetin ≤4.0% (Lera le lesesaane)

Palo yohle ya dibaktheria tsa aerobic ≤103cfu/g

Palo yohle ya hlobo le tomoso ≤102cfu/g

Escherichia coli e ke ke ea fumanoa /g

Salmonella e ke ke ea fumanoa /g

Boemo ba polokelo bo koetsoe 'me bo bolokiloe hole le khanya.

Phello ea meriana:

Ketso ea antifree radical

Limolek'hule tsa oksijene lia fokotseha ka mokhoa oa lielektrone tse le 'ngoe metabolism ea sele, 'me li-ion tsa O tse hlahisoang ke phokotso ea limolek'hule tsa oksijene ka mokhoa oa lielektrone tse le 'ngoe li tla hlahisa H2O2 le li-free radical tse chefo haholo ·OH 'meleng, ka hona li ama boreledi ba letlalo esita le ho potlakisa botsofali ba letlalo. Ho feta moo, ho eketsa rutin sehlahisoa ho ka tlosa mefuta ea oksijene e arabelang e hlahisoang ke lisele. Rutin ke motsoako oa flavonoid, e leng antioxidant e matla bakeng sa ho tlosa li-free radical. E ka emisa karabelo ea ketane ea li-free radical, ea thibela peroxidation ea li-acid tse mafura tse ngata tse saturated ho biofilms, ea tlosa lihlahisoa tsa peroxidation ea lipid, ea sireletsa botšepehi ba li-biofilms le mehaho e ka tlas'a sele, 'me ea bapala karolo ea bohlokoa 'meleng. [2]

Ho thibela peroxidation ea mafura

Peroxidation ea lipid e bolela letoto la lits'ebetso tsa oxidation tse bakoang ke mefuta ea oksijene e arabelang e hlaselang li-acid tse mafura a polyunsaturated ka har'a biofilms. Zhu Jianlin le ba bang. ba fumane le ho sekaseka ts'ebetso ea SOD ho likhoto, litaba tsa sehlahisoa sa free-radical lipid peroxidation MDA, le litaba tsa Lipofuscin sebeteng se seholo, 'me ba fumane hore rutin e na le phello e thibelang peroxidation ea lipid ho likhoto tse lahlehileng, 'me e ka thibela ho fokotseha ha bokhoni ba antioxidant ba sistimi ea antioxidant ho likhoto kamora ho lahleha. Rutin e ka hanela ho fokotseha ha bokhoni ba antioxidant bo bakoang ke ho fokotseha ha estrogen ea tlhaho 'me e na le phello ea antioxidant. High density lipoprotein (HDL) e na le litlamorao tse khahlanong le atherosclerotic. Leha ho le joalo, HDL, joalo ka low density lipoprotein (LDL) le very low density lipoprotein (VLDL), e ka boela ea oxidation le ho fetoloa ka vitro. Hang ha HDL e oxidation le ho fetoloa ho Ox-HDL, e na le phello ea atherosclerosis. Meng Fang le ba bang ba batlisisitse phello ea rutin ho HDL oxidation modification ka Cu2+ mediated oxidative modification in vitro. Qeto Rutin e ka thibela haholo phetoho ea oxidative ea HDL. [2]

Tšusumetso e khahlanong le ntho e sebetsang ea platelet

Pathogenesis ea mafu a mangata a pelo le methapo ea mali, a kang thrombosis, atherosclerosis, karabelo ea ho ruruha le ischemia-reperfusion free radical injury, li amana haufi-ufi le ho kenella ha plateletactivatingfactor (PAF). Ka hona, ho hanyetsa phello ea PAF ke tsela ea bohlokoa ea ho fokotsa mafu a ischemic a pelo le methapo ea mali. Liteko li bontšitse hore rutin e ka hanyetsa tlamo e itseng ea PAF ho li-receptor tsa platelet tsa mmutla tse itšetlehileng ka mahloriso, ea thibela ho khomarela ha platelet ho mebutlanyana le keketseho ea mahloriso a mahala a Ca2+ ho PMNs, e leng se bontšang hore mokhoa oa ketso ea rutin khahlanong le PAF ke ho thibela ts'ebetso ea receptor ea PAF, ebe e thibela karabelo e bakoang ke PAF, e le ho bapala karolo ts'ireletsong ea pelo le methapo ea mali. Liphetho li bontšitse hore rutin e ne e le mohanyetsi oa PAF receptor. [2]

Phekolo ea pancreatitis e matla

Rutin e ka thibela hypocalcemia ka katleho le ho fokotsa mahloriso a Ca2+ liseleng tsa pancreatic. Ho fumanoe hore rutin e ka eketsa litaba tsa phospholipase A2 (PLA2) liseleng tsa pancreatic tsa litoeba, e leng se bontšang hore rutin e ka thibela ho lokolloa le ho kengoa tšebetsong ha PLA2 liseleng tsa pancreatic. Rutin e ka thibela ka katleho ho hlaha ha hypocalcemia litoeng tsa AP, mohlomong ka ho thibela ho kena ha Ca2+ le ho fokotsa ho ja Ca2+ liseleng tsa pancreatic, ka hona ho fokotsa tšenyo ea pathophysiological ho AP. [2]

Referense: https://mp.weixin.qq.com

https://xueshu.baidu.com/usercenter/paper

Bakeng sa rutin, ka kopo ikopanye le rona. Re u emetse mona neng kapa neng.