Rutinformula ea lik'hemik'hale ke (C27H30O16 • 3H2O), vithamine, e na le phello ea ho fokotsa ho phunyeha ha capillary le brittleness, ho boloka le ho tsosolosa elasticity e tloaelehileng ea li-capillaries. Bakeng sa thibelo le kalafo ea hypertensive cerebral haemorrhage; Lefu la tsoekere la retinal hemorrhage le hemorrhagic purpura le tsona li sebelisoa e le li-antioxidants le li-pigments tsa lijo.

E arotsoe ka lintlha tse 'nè tse latelang:

1. Rutin NF11: phofo e mosehla-botala, kapa kristale e ntle haholo ea acicular; Ha e na monko, e sa latsoeheng; Mmala oa fifala moeeng; E futhumetse ho 185-192 ℃, e fetoha 'mele o sootho oa gelatinous mme e bola hoo e ka bang 215 ℃. E qhibiliha hanyenyane ha e belisoa ethanol, e qhibiliha hanyenyane metsing a belang, e qhibiliha hanyenyane haholo metsing a batang, e qhibilihang habonolo ka isopropyl joala le methanol, e sa qhibilihe ka har'a trichloromethane, ether le benzene; E qhibiliha ka tharollo ea alkali hydroxide. Mokhoa oa ho tsebahatsa ke A: hydrochloric acid reflux hydrolysis ho quercetin, eo ntlha ea eona e qhibilihang e leng 312 ℃B: pula e khubelu ea cuprous oxide. C: Ho eketsa tharollo ea sodium hydroxide ke 'mala oa lamunu D: tharollo ea ethanol le ferric chloride solution e sootho e tala E: tharollo ea ethanol e nang le hydrochloric acid le magnesium butle-butle e khubelu: ≥95.0%(UV)(ka lihlahisoa tse omeletseng)

Ho fokotsa boima ba 'mele: 5.5% ~ 9.0%

Masala a tukang ≤0.5%

Chlorophyll ≤0.004%

Pigment e khubelu ≤0.004%

Lintho tse amanang le quercetin ≤5.0%(UV)

Palo eohle ea libaktheria tsa aerobic ≤103cfu/g

Kakaretso ea palo ea hlobo le tomoso ≤102cfu/g

Escherichia coli e ke ke ea fumanoa /g

Maemo a polokelo Boloka ka har'a setshelo se sa keneng moea hole le khanya.

2. Rutoside Trihydrate EP 9.0: Phofo e mosehla kapa e mosehla-botala. E batla e sa qhibilihe ka metsing, e qhibiliha ka methanol, e qhibiliha hanyenyane ka ethanol (96%), e batlang e sa qhibilihe ka har'a methylene chloride. E qhibiliha ka tharollo ea hydroxide. Mokhoa oa ho tsebahatsa ke ka tsela e latelang: A: Ho monya ho hoholo ho 257nm le 358nm, 'me boholo ba coefficient ea ho monya ho 358nm ke 305 ~ 330. boholo bo tla hlaha sebakeng se lumellanang sa chromatogram ea sehlahisoa sa D: tharollo ea ethanol e nang le hydrochloric acid le zinki e tla bontša bofubelu.

Content 95.0% ~ 101.0% (ka sehlahisoa se omeletseng)(titration)

Mongobo 7.5% ~ 9.5%(Cartesian)

Masala a tukang ≤0.1%

Boleng bo phahameng ba ho monya leseli la litšila tsa optical ho 450nm ho isa ho 800nm ​​ha boa lokela ho feta 0.10

Lintho tse sa qhibiliheng ka methanol ≤3.0%

Ntho e amanang le isoquercetin ≤2.0%, kaempferol-3-rutin ≤2.0%, quercetin ≤2.0%, ho se hloeke hohle ≤4.0%(HPLC)

Palo eohle ea libaktheria tsa aerobic ≤104cfu/g

Kakaretso ea palo ea hlobo le tomoso ≤102cfu/g

Bile gram-negative baktheria ≤102cfu/g

Escherichia coli e ke ke ea fumanoa /g

Salmonella e kanna ea se ke ea bonoa / 25g

Maemo a polokelo a boloka hole le khanya

3. Rutin USP43: Mokhoa oa ho tsebahatsa ke A: ho monya ho hoholo ho 257nm le 358nm, le coefficient e phahameng ea ho monya ho 358nm ke 305 ~ 33. B: Pono ea infrared e lokela ho lumellana le chromatogram ea sehlahisoa sa litšupiso. C: Nako ea ho boloka tlhoro ea chromatogram e lokela ho lumellana le ea sehlahisoa sa litšupiso

Content 95.0% ~ 101.0% (ka sehlahisoa se omeletseng)(titration)

Mongobo 7.5% ~ 9.5%(Cartesian)

Masala a tukang ≤0.1%

Boleng bo phahameng ba ho monya leseli la litšila tsa optical ho 450nm ho isa ho 800nm ​​ha boa lokela ho feta 0.10

Lintho tse sa qhibiliheng ka methanol ≤3.0%

Lintho tse amanang le isoquercetin ≤2.0%, kaempferol-3-rutin ≤2.0%, quercetin ≤2.0%, tse ling tse mono-miscellaneous ≤1.0%, ho se hloeke hohle ≤4.0%(HPLC)

Palo eohle ea libaktheria tsa aerobic ≤104cfu/g

Kakaretso ea palo ea hlobo le tomoso ≤103cfu/g

Escherichia coli e ke ke ea fumanoa ka 10g

Salmonella e ke ke ea fumanoa ka 10g

Boemo ba polokelo bo tiisitsoe le ho bolokoa hole le khanya.

4. Tekanyetso ea Lekala WS1-49(B)-89 ea Rutinum: phofo e mosehla kapa e mosehla-botala, kapa kristale e ntle haholo ea acicular; Ha e na monko, e sa latsoeheng; Mmala oa fifala moeeng; E futhumetse ho 185 ~ 192 ℃ ho fetoha gel e sootho. E qhibiliha hanyenyane ha e belisoa ethanol, e qhibiliha hanyenyane metsing a belang, e qhibiliha hanyenyane haholo metsing a batang, e ke keng ea qhibiliha ka trichloromethane le ether; E qhibiliha ka tharollo ea alkali hydroxide. Mokhoa oa ho tsebahatsa ke: A: red cuprous oxide precipitate. B: Mokhoa oa ho monya ha infrared o lokela ho lumellana le oa ntho ea taolo. C: Ho monya ho hoholo ho fumanoa makhubung a 259±1nm le 362.5±1nm.

Content ≥93.0%(UV)(ka sehlahisoa se omeletseng)

Ho lahleheloa ke boima ba 'mele 5.5% ~ 9.0%

Masala a tukang ≤0.3%

Ntho e sa qhibiliheng ka har'a methanol ≤2.5% (ntho e sa tsitsang ka ethanol)

Lintho tse amanang le quercetin ≤4.0%(Thin layer)

Palo eohle ea libaktheria tsa aerobic ≤103cfu/g

Kakaretso ea palo ea hlobo le tomoso ≤102cfu/g

Escherichia coli e ke ke ea fumanoa /g

Salmonella e ke ke ea fumanoa /g

Boemo ba polokelo bo tiisitsoe le ho bolokoa hole le khanya.

Pharmacological effect:

Ketso e matla ea antifree

Limolek'hule tsa oksijene li fokotsehile ka sebopeho sa lielektrone tse le 'ngoe ho metabolism ea lisele,' me li-ion tsa O tse hlahisoang ke phokotso ea limolek'hule tsa oksijene ka sebopeho sa lielektrone tse le 'ngoe li tla hlahisa H2O2 le chefo e ngata ·OH mahala radicals' meleng, kahoo e ama letlalo. boreleli esita le ho potlakisa ho tsofala ha letlalo. Ho feta moo, ho eketsa rutin ho sehlahisoa ho ka tlosa mefuta ea oksijene e sebetsang e hlahisoang ke lisele. Rutin ke motsoako oa flavonoid, e leng antioxidant e matla bakeng sa ho senya li-radicals tsa mahala. E ka emisa karabelo ea ketane ea li-radicals tsa mahala, e thibela peroxidation ea li-polyunsaturated fatty acids ho li-biofilms, ea tlosa lihlahisoa tsa lipid peroxidation, e sireletsa botšepehi ba li-biofilms le mehaho ea subcellular, le ho phetha karolo ea bohlokoa 'meleng. [2]

Anti-lipid peroxidation

Lipid peroxidation e bolela letoto la lits'ebetso tsa oxidation tse bakoang ke mefuta e sebetsang ea oksijene e hlaselang mafura a polyunsaturated a li-biofilms. Zhu Jianlin et al. e ikemiselitse le ho sekaseka ts'ebetso ea SOD ho likhoto, litaba tsa sehlahisoa sa MDA sa mahala-radical lipid peroxidation, le litaba tsa Lipofuscin ka har'a sebete se seholo, mme a fumana hore rutin e na le phello e thibelang ho lipid peroxidation ho likhoto tse khaotsoeng, mme e ka thibela ho fokotseha ha matla a antioxidant. ea tsamaiso ea li-antioxidant ho likhoto ka mor'a ho haneloa. Rutin e ka hanana le ho fokotseha ha matla a antioxidant a bakoang ke ho fokotseha ha endogenous estrogen mme e na le phello ea antioxidant. High density lipoprotein (HDL) e na le litlamorao tse khahlanong le atherosclerotic. Leha ho le joalo, HDL, joalo ka low density lipoprotein (LDL) le low density lipoprotein (VLDL) le eona e ka etsoa oxidized le ho fetoloa in vitro. Hang ha HDL e se e entsoe ka oxidized 'me e fetotsoe ho Ox-HDL, e na le phello ea atherosclerosis. Meng Fang et al. e ile ea etsa lipatlisiso ka phello ea rutin ho HDL oxidative modification ka Cu2+ mediated oxidative modification in vitro. Qetello Rutin e ka thibela haholo phetoho ea oxidative ea HDL. [2]

Phello e hanyetsanang ea platelet activating factor

Pathogenesis ea mafu a mangata a pelo le cerebrovascular, joalo ka thrombosis, atherosclerosis, inflammatory reaction le ischemia-reperfusion free radical injury, li amana haufi-ufi le ho kena lipakeng ha plateletactivatingfactor (PAF). Ka hona, ho hanyetsa phello ea PAF ke mokhoa oa bohlokoa oa ho fokotsa mafu a pelo le methapo ea pelo. Liteko li bonts'itse hore rutin e ka thibela tlamahano e khethehileng ea PAF ho li-receptors tse itšetlehileng haholo ka liplatelete tsa mmutla, tsa thibela ho khomarela ha liplatelete tsa PAf ho mebutlanyana le ho eketseha ha mahloriso a mahala a Ca2+ ho PMNs, a fana ka maikutlo a hore mochine oa rutin o khahlanong le PAF. ho thibela ts'ebetso ea PAF receptor, ebe e thibela karabelo e bakoang ke PAF, e le ho phetha karolo ea ts'ireletso ea pelo. Liphetho li bontšitse hore rutin e ne e le mohanyetsi oa PAF receptor. [2]

Anti-acute pancreatitis

Rutin e ka thibela hypocalcemia ka katleho le ho fokotsa khatello ea Ca2+ liseleng tsa pancreatic. Ho fumanoe hore rutin e ka eketsa litaba tsa phospholipase A2 (PLA2) ka har'a litoeba tsa pancreatic, ho fana ka maikutlo a hore rutin e ka thibela ho lokolloa le ts'ebetso ea PLA2 liseleng tsa pancreatic. Rutin e ka thibela ka katleho ketsahalo ea hypocalcemia ho likhoto tsa AP, mohlomong ka ho thibela phallo ea Ca2 + le ho fokotsa boima ba Ca2 + liseleng tsa lisele tsa pancreatic, ka hona ho fokotsa tšenyo ea pathophysiological ho AP. [2]

Tlhaloso: https://mp.weixin.qq.com

https://xueshu.baidu.com/usercenter/paper

Bakeng sa rutin, ka kopo ikopanye le rona. Re u emetse mona ka nako efe kapa efe.